mcpC [2019-08-28 09:35:51]

membrane-bound chemotaxis receptor for proline, methyl-accepting chemotaxis protein

locus: BSU_13950

pI: 5.17

mw: 71.93 kDa

protein length: 655 aa

gene length: 1968 bp

function: control of chemotaxisthreonine, glycine, serine, lysine, valine and arginine

product: methyl-accepting chemotaxis protein

essential: no

synonyms: prg71

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.4|Membrane-bound chemoreceptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,463,628 1,465,595

The protein

Catalyzed reaction/ biological activity

• deaminated [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|McpC] stimulates phosphorylation of [protein|6B3B222E56BF0C95A2371CA5208B5522B44D4689|CheA] [Pubmed|22931217]

Modification

• deamination of Gln-609 (by [protein|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|CheD]) [Pubmed|22931217], deaminated [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|McpC] stimulates phosphorylation of [protein|6B3B222E56BF0C95A2371CA5208B5522B44D4689|CheA] [Pubmed|22931217]

[SW|Localization]

• cell membrane [Pubmed|18763711,21515776]

Expression and Regulation

Operons

• genes: [gene|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]
  description: [Pubmed|9353924]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|9353924], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, McpC is present with 2,800 /- 640 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• DS180 (''mcpC''::''mls'' in NCIB3610) [Pubmed|12864845]
• BKE13950 ([gene|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCAATCCCCTCCTAAT, downstream forward: _UP4_TAATCCAATTACATCCCCAA
• BKK13950 ([gene|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCAATCCCCTCCTAAT, downstream forward: _UP4_TAATCCAATTACATCCCCAA

References

• 12864845,15317802,8251536,9353924,15544802,6137212,9721285,2505839,2105313,12603740,23038252,18763711,9721285,21515776,22931217,27899502